Ces bactéries ont été isolées dans des zones côtières au Japon et principalement en hiver. Une analyse du polymorphisme de la longueur des fragments de restriction (RFLP) a été faite sur 136 isolats naturels appartenant à la famille des Vibrionaceae. Key words: Vibrio, vibrios, psychrotroph, restriction fragment length polymorphism (RFLP) analysis, Vibrio splendidus. It suggests that many psychrotrophic vibrios in cold marine environments remain as unknown species. Most of the isolates studied were not identified as previously described species. From the clustering analysis, it was concluded that group V strains were not Vibrio species. Group V strains showed RFLP patterns which have not been observed previously. The RFLP profile indicated that Group IV strains were closely related to V. Group III strains were regarded as members of the Vibrio main group, which is a major phylogenetic group deduced from 16S rRNA gene analysis in the family Vibrionaceae. Group II strains were not identified as previously known Vibrio species. It was confirmed that this group was not only found in Otsuchi Bay, but also in broad coastal areas of Japan. Group I corresponded to Vibrio splendidus-like strains. Five RFLP groups (groups I to V) were obtained. A dendrogram was constructed using the unweighted pair group method with the arithmetic averages algorithm. To estimate the genetic relationships, 53 informative fragments were scored by their presence or absence. Twenty-eight 16S rDNA genotypes were obtained by digestion with four restriction endonucleases ( HhaI, DdeI, RsaI, and Sau3AI). These were collected from inshore areas of Japan, mainly in winter. Restriction fragment length polymorphism (RFLP) analysis was carried out for 136 natural isolates belonging to the family Vibrionaceae.
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